3）按你课题定制的“一页总览技术路线图”（文字版，可直接照着画） ① 模型构建（输入/平台） 供体...

Designing a technical roadmap layout

I’m thinking of a design for the roadmap. The top section should say "技术路线图" with a vertical flow. I'll have a big box for [模型构建] with sub-parts like donor, recipient, QCs, etc., then arrows flowing down to different goals like [目标1: 表型解析]. I'll arrange the elements vertically but allow side branches for clarity. The deliverables should be at the far right. Also, highlighting the "20% Jak2V617F" in the modeling frame is key.

Designing technical roadmap for mechanistic controls

I need to show differential controls: WT→Ldlr-/- vs Jak2V617F→Ldlr-/- and groups with/without rapamycin. I'll also illustrate the mechanistic chain, starting from Jak2V617F → mTOR/HIF-1α → metabolic reprogramming → NETs/inflammation → plaque burden and stability. I’ll plan a layout with short explanations and capture key readouts like phenotype, mechanism, and intervention effects. I'll highlight the CHIP mimic at 20% chimera.

Designing a succinct layout for the technical model

I need to provide an ASCII layout with pipes and arrows showing the model construction. Each box should have short, succinct points, like 4-6 lines at most. Labels for control and Jak2 branches should be clear, and I'll mark critical aspects such as CHIMERA, pathway enrichment, and short-term drug treatment. I'll explicitly include key readouts like mTOR readouts and relevant labels to capture everything the user values.

Designing a clear model layout

I’m thinking of creating a clean, vertical layout with the deliverables at the far right, as requested. Each box will have 4–5 short lines, with 6–10 characters per line. I’ll include titles like "模型构建" for each section and use arrows pointing downward. The deliverables column will stretch to the right. I'll keep it minimal and concise to match their instructions.

Designing a succinct model layout

I’m creating a step-by-step, clear design with concise phrases. For modeling, I’ll organize it into boxes (A, B, C, D). Each box will focus on specifics: For example, Box A will highlight control groups and model details, Box B will focus on phenotype analysis, Box C on mechanism analysis, and Box D on intervention and reversibility. I'll also incorporate key points, such as 20% chimerism and causal evidence, as explicitly marked in the layout.

Clarifying components for roadmap and final diagram

They asked for a technical roadmap, focusing on key points for design. It’s important to stick to the 5 "加分点" we see, but I’ll add a note outside the diagram if they want added context (like showing where the key points fit within the boxes). I’ll keep the text concise and clear, as requested.

Designing concise box content for technical roadmap

We need to keep the layout clean and concise, respecting the user's request for short phrases within each bracket. The yellow highlight for important points like "亮点" is fine, so for the model box, I'll keep the following lines:

• 供体：Mx1-Cre;Jak2V617F 骨髓（CD45/GFP）
• 受体：WT 或 Ldlr-/- 小鼠
• 嵌合：全量 vs 20% Jak2V617F 嵌合（CHIP模拟）
• 诱导：poly(I:C) 激活 Jak2V617F
• 饮食：WTD 7 周→粥样斑块
• 质控：嵌合率及血常规检查

This should fit well within the box while still providing the necessary details clearly!

Designing concise layout for technical roadmap

For clarity, the control arms can be integrated into Box 1 with clear labels for both treatment and control groups. I'll keep lines concise in each box and ensure the terminology is accurate:

Box 1:

• 供体：Mx1-Cre;Jak2V617F 骨髓（CD45/GFP）
• 受体

Thought for 32s